Current biomedical research and diagnostics critically depend on detection agents for specific recognition and quantification of protein molecules. Monoclonal antibodies have been used for this purpose over decades and facilitated numerous biological and biomedical investigations. Recently, however, it has become apparent that many commercial reagent antibodies lack specificity or do not recognize their target at all. Thus, synthetic alternatives are needed whose complex designs are facilitated by multidisciplinary approaches incorporating experimental protein engineering with computational modeling. Here, we review the status of such an engineering endeavor based on the modular armadillo repeat protein scaffold and discuss challenges in its implementation.
Bibliographical note© 2022 Florian J. Gisdon et al., published by De Gruyter. This work is licensed under the Creative Commons Attribution 4.0
Funding: This work was supported by H2020-FETopen-RIA grant agreement 764434 (‘PRe-ART’)
- Clinical Biochemistry
- Molecular Biology