Protein and peptides in pictures: imaging with MALDI mass spectrometry

Richard J.A. Goodwin, Stephen R. Pennington, Andrew R. Pitt

Research output: Contribution to journalArticle

Abstract

Imaging using MS has the potential to deliver highly parallel, multiplexed data on the specific localization of molecular ions in tissue samples directly, and to measure and map the variations of these ions during development and disease progression or treatment. There is an intrinsic potential to be able to identify the biomarkers in the same experiment, or by relatively simple extension of the technique. Unlike many other imaging techniques, no a priori knowledge of the markers being sought is necessary. This review concentrates on the use of MALDI-MS for MS imaging (MSI) of proteins and peptides, with an emphasis on mammalian tissue. We discuss the methodologies used, their potential limitations, overall experimental considerations and progress that has been made towards establishing MALDI-MSI as a routine technique for the spatially resolved measurement of peptides and proteins. As well as determining the local abundance of individual molecular ions, there is the potential to determine their identity within the same experiment using relatively simple extensions of the basic techniques. In this way MSI offers an important opportunity for biomarker discovery and identification.
LanguageEnglish
Pages3785-3800
Number of pages16
JournalProteomics
Volume8
Issue number18
Early online date19 Aug 2008
DOIs
Publication statusPublished - 18 Sep 2008

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mass spectrometry
Mass Spectrometry
Ions
Imaging techniques
Peptides
Biomarkers
Proteins
Disease Progression
Tissue
Experiments

Keywords

  • animals
  • biological markers
  • humans
  • peptide mapping
  • peptides
  • proteins
  • spectrometry
  • tissue preservation
  • mass
  • matrix-assisted laser desorption-ionization

Cite this

Goodwin, Richard J.A. ; Pennington, Stephen R. ; Pitt, Andrew R. / Protein and peptides in pictures : imaging with MALDI mass spectrometry. In: Proteomics. 2008 ; Vol. 8, No. 18. pp. 3785-3800.
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Protein and peptides in pictures : imaging with MALDI mass spectrometry. / Goodwin, Richard J.A.; Pennington, Stephen R.; Pitt, Andrew R.

In: Proteomics, Vol. 8, No. 18, 18.09.2008, p. 3785-3800.

Research output: Contribution to journalArticle

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