Using an in vitro human airway model to study the toxic effects of components of e-cigarettes

Pranav Vasanthi Narayanan, L.J. Leslie, L.J. Marshall

Research output: Contribution to conferencePoster

Abstract

Cigarette smoke is a complex mixture of more than 4000 hazardous chemicals including the carcinogenic benzopyrenes. Nicotine, the most potent component of tobacco, is responsible for the addictive nature of cigarettes and is a major component of e-cigarette cartridges.
Our study aims to investigate the toxicity of nicotine with special emphasis on the replacement of animals. Furthermore, we intend to study the effect of nicotine, cigarette smoke and e-cigarette vapours on human airways. In our current work, the BEAS 2B human bronchial epithelial cell line was used to analyse the effect of nicotine in isolation, on cell viability. Concentrations of nicotine from 1.1µM to 75µM were added to 5x105 cells per well in a 96 well plate and incubated for 24 hours.
Cell titre blue results showed that all the nicotine treated cells were more metabolically active than the control wells (cells alone). These data indicate that, under these conditions, nicotine does not affect cell viability and in fact, suggests that there is a stimulatory effect of nicotine on metabolism. We are now furthering this finding by investigating the pro-inflammatory response of these cells to nicotine by measuring cytokine secretion via ELISA.
Further work includes analysing nicotine exposure at different time points and on other epithelial cells lines like Calu-3.
Original languageEnglish
Publication statusPublished - 27 Nov 2014
EventAnimal Replacement Science 2014 - Charles Darwin House, London, United Kingdom
Duration: 27 Nov 2014 → …

Conference

ConferenceAnimal Replacement Science 2014
CountryUnited Kingdom
CityLondon
Period27/11/14 → …
OtherImproving relevance to human disease – challenges, innovations and applications

Fingerprint

Poisons
Nicotine
Tobacco Products
Smoke
Cell Survival
Benzopyrenes
Epithelial Cells
In Vitro Techniques
Hazardous Substances
Cell Line
Complex Mixtures
Tobacco
Enzyme-Linked Immunosorbent Assay
Cytokines

Cite this

Vasanthi Narayanan, P., Leslie, L. J., & Marshall, L. J. (2014). Using an in vitro human airway model to study the toxic effects of components of e-cigarettes. Poster session presented at Animal Replacement Science 2014, London, United Kingdom.
Vasanthi Narayanan, Pranav ; Leslie, L.J. ; Marshall, L.J. / Using an in vitro human airway model to study the toxic effects of components of e-cigarettes. Poster session presented at Animal Replacement Science 2014, London, United Kingdom.
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Vasanthi Narayanan, P, Leslie, LJ & Marshall, LJ 2014, 'Using an in vitro human airway model to study the toxic effects of components of e-cigarettes' Animal Replacement Science 2014, London, United Kingdom, 27/11/14, .

Using an in vitro human airway model to study the toxic effects of components of e-cigarettes. / Vasanthi Narayanan, Pranav; Leslie, L.J.; Marshall, L.J.

2014. Poster session presented at Animal Replacement Science 2014, London, United Kingdom.

Research output: Contribution to conferencePoster

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T1 - Using an in vitro human airway model to study the toxic effects of components of e-cigarettes

AU - Vasanthi Narayanan, Pranav

AU - Leslie, L.J.

AU - Marshall, L.J.

PY - 2014/11/27

Y1 - 2014/11/27

N2 - Cigarette smoke is a complex mixture of more than 4000 hazardous chemicals including the carcinogenic benzopyrenes. Nicotine, the most potent component of tobacco, is responsible for the addictive nature of cigarettes and is a major component of e-cigarette cartridges.Our study aims to investigate the toxicity of nicotine with special emphasis on the replacement of animals. Furthermore, we intend to study the effect of nicotine, cigarette smoke and e-cigarette vapours on human airways. In our current work, the BEAS 2B human bronchial epithelial cell line was used to analyse the effect of nicotine in isolation, on cell viability. Concentrations of nicotine from 1.1µM to 75µM were added to 5x105 cells per well in a 96 well plate and incubated for 24 hours.Cell titre blue results showed that all the nicotine treated cells were more metabolically active than the control wells (cells alone). These data indicate that, under these conditions, nicotine does not affect cell viability and in fact, suggests that there is a stimulatory effect of nicotine on metabolism. We are now furthering this finding by investigating the pro-inflammatory response of these cells to nicotine by measuring cytokine secretion via ELISA.Further work includes analysing nicotine exposure at different time points and on other epithelial cells lines like Calu-3.

AB - Cigarette smoke is a complex mixture of more than 4000 hazardous chemicals including the carcinogenic benzopyrenes. Nicotine, the most potent component of tobacco, is responsible for the addictive nature of cigarettes and is a major component of e-cigarette cartridges.Our study aims to investigate the toxicity of nicotine with special emphasis on the replacement of animals. Furthermore, we intend to study the effect of nicotine, cigarette smoke and e-cigarette vapours on human airways. In our current work, the BEAS 2B human bronchial epithelial cell line was used to analyse the effect of nicotine in isolation, on cell viability. Concentrations of nicotine from 1.1µM to 75µM were added to 5x105 cells per well in a 96 well plate and incubated for 24 hours.Cell titre blue results showed that all the nicotine treated cells were more metabolically active than the control wells (cells alone). These data indicate that, under these conditions, nicotine does not affect cell viability and in fact, suggests that there is a stimulatory effect of nicotine on metabolism. We are now furthering this finding by investigating the pro-inflammatory response of these cells to nicotine by measuring cytokine secretion via ELISA.Further work includes analysing nicotine exposure at different time points and on other epithelial cells lines like Calu-3.

M3 - Poster

ER -

Vasanthi Narayanan P, Leslie LJ, Marshall LJ. Using an in vitro human airway model to study the toxic effects of components of e-cigarettes. 2014. Poster session presented at Animal Replacement Science 2014, London, United Kingdom.