1989 …2020

Research output per year

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Personal profile

General

I graduated in Natural Sciences from Cambridge University in 1981and then stayed to do a Ph.D in the pharmacology department under the supervision of Prof. Sir Arnold Burgen. I subsequently did post-doctoral work at the National Institute for Medical Research, working with Drs Ed Hulme and Nigel Birdsall and then at the MRC Molecular Neurobiology Unit in Cambridge with Dr Mike Hanley. It was here that I first started to work on the pharmacology of calcitonin gene related peptide (CGRP). I was appointed to a lectureship at Aston University in 1991 where I have continued my work on this and allied peptides.

Research Interests

My chief interest is in receptors for neuropeptides, especially those for calcitonin gene-related peptide (CGRP) and adrenomedullin. CGRP is a very abundant 37 amino acid peptide with many actions ranging from vasodilation to inhibition of some of the effects of insulin on metabolism. Adrenomedullin is a related peptide; it plays a very important role in the cardiovascular system. Drugs developed from CGRP and adrenomedullin may be of benefit in a variety of conditions such as migraine, heart disease and arthritis.

Both CGRP and adrenomedullin produce their effects at G-protein coupled receptors (GPCRs). Something like 70% of all drugs act at GPCRs; thus this family is of particular interest in drug discovery. However, the receptors for CGRP and adrenomedullin are of especial interest as they are made up of two subunits; a most unusual arrangement for GPCRs. They share a common subunit called calcitonin receptor-like receptor (CRLR or CL). This has the structure of a typical GPCR with seven transmembrane helices. However, to respond to CGRP a second protein is required, called receptor activity modifying protein 1 (RAMP1). When CL complexes with the related proteins RAMP2 or RAMP3, adrenomedullin receptors are formed.

In my laboratory we are interested in the molecular and pharmacological characterisation of CGRP and adrenomedullin receptors. Thus we wish to discover how CGRP and adrenomedullin bind to their receptors, how the receptors then activate the cells, how drugs discriminate between these receptors and what other receptors CGRP and adrenomedullin can activate besides CL/RAMP complexes.

We use a variety of techniques involving mutating receptors and expressing these in cell lines to measure binding and activation. We also look at endogenous receptors in cells and tissues. We collaborate with colleagues at Cambridge, Coventry, Essex and Birmingham Universities and other institutions in the UK and overseas to further these studies.

Teaching Activity

I specialise in the teaching of molecular pharmacology, especially cell receptors and signal transduction. I also teach general pharamacology and physiology, cell biology and biochemistry. I teach in Pharmacy, Neuroscience, Biology and Medicine at all levels of these programme and I am head of the pharmacology teaching group.

Main modules taught:

  • Pharmacology (especially PH2509)
  • Biochemistry (especially NE1009 and BY1BC1)
  • Physiology (especially PH1401)

Employment

  • 2012 to date Professor in Pharmacology, Aston University
  • 2005 – 2012 Reader Pharmacology, Aston University
  • 2000 – 2005 Senior Lecturer in Pharmacology, Aston University
  • 1991 – 2000 Lecturer in Pharmacology, Aston University
  • 1988 – 1991 MRC Short Term Staff Scientist, MRC Molecular Neurobiology Unit, Laboratory of Molecular Biology, Cambridge
  • 1985 – 1988 MRC Training Fellow, National Institute for Medical Research, London

Qualifications

  • 1982 - 1985 PhD University of Cambridge

  • 1978 - 1982 BA, Pharmacology (Class I), University of Cambridge

Membership of Professional Bodies

British Pharmacological Society

Biochemical Society

Responsibilities

Head of Pharmacology Teaching Group

Director of Research, Pharmacology and Translational Neuroscience

Funding Applications and Awards

BBSRC, BB/R016755/1, Investigating GPCR:RAMP interactions using nanobodies, 2019-21 (with Prof Mark Wheatley, Coventry University)

 

Contact Details

Emaild.r.poyner@aston.ac.uk

Telephone: +44 (0) 121 204 3997

Fax: +44 (0) 121359 5142

Room: MB438r

External positions

Associate

Keywords

  • RM Therapeutics. Pharmacology
  • G protein coupled receptors
  • CGRP
  • Adrenomedullin
  • QP Physiology

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Research Output

A comparison of SMA (styrene maleic acid) and DIBMA (di-isobutylene maleic acid) for membrane protein purification

Gulamhussein, A., Uddin, R., Tighe, B., Poyner, D. & Rothnie, A., 1 Jul 2020, In : BBA -Biomembranes. 1862, 7, 183281.

Research output: Contribution to journalArticle

  • Calcitonin receptor N-glycosylation enhances peptide hormone affinity by controlling receptor dynamics

    Lee, S., Jeong, Y., Simms, J., Warner, M. L., Poyner, D. R., Chung, K. Y. & Pioszak, A. A., 27 Mar 2020, In : Journal of Molecular Biology . 432, 7, p. 1996-2014 19 p.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Expression and purification of recombinant G protein-coupled receptors: A review

    Wiseman, D. N., Otchere, A., Patel, J. H., Uddin, R., Pollock, N. L., Routledge, S. J., Rothnie, A. J., Slack, C., Poyner, D. R., Bill, R. M. & Goddard, A. D., 1 Mar 2020, In : Protein Expression and Purification. 167, 105524.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Ligand-induced conformational changes in a SMALP-encapsulated GPCR.

    Routledge, S. J., Jamshad, M., Little, H. A., Lin, Y. P., Simms, J., Thakker, A., Spickett, C. M., Bill, R. M., Dafforn, T. R., Poyner, D. R. & Wheatley, M., 1 Jun 2020, In : Biochimica et Biophysica Acta - Biomembranes. 1862, 6, 183235.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Receptor component protein, an endogenous allosteric modulator of family B G protein coupled receptors

    Routledge, S. J., Simms, J., Clark, A., Yeung, H. Y., Wigglesworth, M. J., Dickerson, I. M., Kitchen, P., Ladds, G. & Poyner, D. R., 1 Mar 2020, In : BBA -Biomembranes. 1862, 3, 183174.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Datasets

    Effects of receptor component protein on family B GPCRs

    Routledge, S. (Creator), Poyner, D. (Creator), Ladds, G. (Creator), Clark, A. (Creator) & Yeung, H. H. (Creator), Aston Data Explorer, 9 Dec 2019

    Dataset

    Activities

    • 2 Visiting an external academic institution
    • 1 Participation in conference

    University of Rochester

    David Poyner (Visiting researcher)

    2011 → …

    Activity: Visiting an external institution typesVisiting an external academic institution

    University of Auckland

    David Poyner (Visiting researcher)

    2011 → …

    Activity: Visiting an external institution typesVisiting an external academic institution

    7th International Meeting on CGRP

    David Poyner (Member of programme committee)

    28 Aug 201031 Aug 2011

    Activity: Participating in or organising an event typesParticipation in conference