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Personal profile

General

I graduated in Natural Sciences from Cambridge University in 1981and then stayed to do a Ph.D in the pharmacology department under the supervision of Prof. Sir Arnold Burgen. I subsequently did post-doctoral work at the National Institute for Medical Research, working with Drs Ed Hulme and Nigel Birdsall and then at the MRC Molecular Neurobiology Unit in Cambridge with Dr Mike Hanley. It was here that I first started to work on the pharmacology of calcitonin gene related peptide (CGRP). I was appointed to a lectureship at Aston University in 1991 where I have continued my work on this and allied peptides.

Research Interests

My chief interest is in receptors for neuropeptides, especially those for calcitonin gene-related peptide (CGRP) and adrenomedullin. CGRP is a very abundant 37 amino acid peptide with many actions ranging from vasodilation to inhibition of some of the effects of insulin on metabolism. Adrenomedullin is a related peptide; it plays a very important role in the cardiovascular system. Drugs developed from CGRP and adrenomedullin may be of benefit in a variety of conditions such as migraine, heart disease and arthritis.

Both CGRP and adrenomedullin produce their effects at G-protein coupled receptors (GPCRs). Something like 70% of all drugs act at GPCRs; thus this family is of particular interest in drug discovery. However, the receptors for CGRP and adrenomedullin are of especial interest as they are made up of two subunits; a most unusual arrangement for GPCRs. They share a common subunit called calcitonin receptor-like receptor (CRLR or CL). This has the structure of a typical GPCR with seven transmembrane helices. However, to respond to CGRP a second protein is required, called receptor activity modifying protein 1 (RAMP1). When CL complexes with the related proteins RAMP2 or RAMP3, adrenomedullin receptors are formed.

In my laboratory we are interested in the molecular and pharmacological characterisation of CGRP and adrenomedullin receptors. Thus we wish to discover how CGRP and adrenomedullin bind to their receptors, how the receptors then activate the cells, how drugs discriminate between these receptors and what other receptors CGRP and adrenomedullin can activate besides CL/RAMP complexes.

We use a variety of techniques involving mutating receptors and expressing these in cell lines to measure binding and activation. We also look at endogenous receptors in cells and tissues. We collaborate with colleagues at Cambridge, Coventry, Essex and Birmingham Universities and other institutions in the UK and overseas to further these studies.

Teaching Activity

I specialise in the teaching of molecular pharmacology, especially cell receptors and signal transduction. I also teach general pharamacology and physiology, cell biology and biochemistry. I teach in Pharmacy, Neuroscience, Biology and Medicine at all levels of these programme and I am head of the pharmacology teaching group.

Main modules taught:

  • Pharmacology (especially PH2509)
  • Biochemistry (especially NE1009 and BY1BC1)
  • Physiology (especially PH1401)

Employment

  • 2012 to date Professor in Pharmacology, Aston University
  • 2005 – 2012 Reader Pharmacology, Aston University
  • 2000 – 2005 Senior Lecturer in Pharmacology, Aston University
  • 1991 – 2000 Lecturer in Pharmacology, Aston University
  • 1988 – 1991 MRC Short Term Staff Scientist, MRC Molecular Neurobiology Unit, Laboratory of Molecular Biology, Cambridge
  • 1985 – 1988 MRC Training Fellow, National Institute for Medical Research, London

Qualifications

  • 1982 - 1985 PhD University of Cambridge

  • 1978 - 1982 BA, Pharmacology (Class I), University of Cambridge

Membership of Professional Bodies

British Pharmacological Society

Biochemical Society

Responsibilities

Head of Pharmacology Teaching Group

Director of Research, Pharmacology and Translational Neuroscience

Funding Applications and Awards

BBSRC, BB/R016755/1, Investigating GPCR:RAMP interactions using nanobodies, 2019-21 (with Prof Mark Wheatley, Coventry University)

 

Research Interests

Emaild.r.poyner@aston.ac.uk

Telephone: +44 (0) 121 204 3997

Fax: +44 (0) 121359 5142

Room: MB438r

Keywords

  • RM Therapeutics. Pharmacology
  • G protein coupled receptors
  • CGRP
  • Adrenomedullin
  • QP Physiology

Fingerprint Dive into the research topics where David Poyner is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 6 Similar Profiles
Calcitonin Gene-Related Peptide Receptors Medicine & Life Sciences
Calcitonin Receptor-Like Protein Medicine & Life Sciences
G-Protein-Coupled Receptors Medicine & Life Sciences
Calcitonin Gene-Related Peptide Medicine & Life Sciences
Receptor Activity-Modifying Proteins Medicine & Life Sciences
Adrenomedullin Medicine & Life Sciences
Receptor Activity-Modifying Protein 1 Medicine & Life Sciences
Adrenomedullin Receptors Medicine & Life Sciences

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Research Output 1989 2019

Expression and purification of recombinant G protein-coupled receptors: A review

Wiseman, D. N., Otchere, A., Patel, J. H., Uddin, R., Pollock, N. L., Routledge, S. J., Rothnie, A. J., Slack, C., Poyner, D. R., Bill, R. M. & Goddard, A. D., 31 Oct 2019, In : Protein Expression and Purification.

Research output: Contribution to journalArticle

Drug Design
Drug Discovery
G-Protein-Coupled Receptors
Recombinant Proteins
Detergents

Interactions Between RAMP2 And CRF Receptors: The Effect Of Receptor Subtypes, Splice Variants And Cell Context

Bailey, S., Harris, M., Barkan, K., Winfield, I., Thomas-Harper, M., Simms, J. W., Ladds, G., Wheatley, M. & Poyner, D. R., 1 May 2019, In : BBA -Biomembranes. 1861, 5, p. 997-1003 7 p.

Research output: Contribution to journalArticle

Corticotropin-Releasing Hormone Receptors
Corticotropin-Releasing Hormone
Calcitonin Receptors
Cell Line
Histidine

Functional characterisation of G protein-coupled receptors

Uddin, R., Simms, J. & Poyner, D., 3 Mar 2018, In : Methods.

Research output: Contribution to journalArticle

Open Access
File
G-Protein-Coupled Receptors
Ligands
Fluorescence Resonance Energy Transfer
GTP-Binding Proteins
Chemical activation

hCALCRL mutation causes autosomal recessive nonimmune hydrops fetalis with lymphatic dysplasia

Mackie, D. I., Al Mutairi, F., Davis, R. B., Kechele, D. O., Nielsen, N. R., Snyder, J. C., Caron, M. G., Kliman, H. J., Berg, J. S., Simms, J., Poyner, D. R. & Caron, K. M., 16 Aug 2018, In : Journal of Experimental Medicine . 215, 9, p. 2339-2353

Research output: Contribution to journalArticle

Open Access
File
Hydrops Fetalis
Mutation
Receptor Activity-Modifying Proteins
Calcitonin Receptor-Like Protein
Fetal Death

Microbial expression systems for membrane proteins

Dilworth, M. V., Piel, M. S., Bettaney, K. E., Ma, P., Luo, J., Sharples, D., Poyner, D. R., Gross, S. R., Moncoq, K., J. F. Henderson, P., Miroux, B. & Bill, R. M., 12 Apr 2018, In : Methods.

Research output: Contribution to journalArticle

Open Access
File
Membrane Proteins
Genetic engineering
Proteins
Recombinant DNA
Crystallization

Thesis

An insight into the activation mechanism of the calcatonin-gene-related peptide receptor

Author: Barwell, J., Feb 2010

Supervisor: Poyner, D. R. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Characterisation of calcitonin generelated peptide (CGRP) and amylin receptors

Author: Tomlinson, A. E., Sep 1995

Supervisor: Poyner, D. R. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Investigation of the activation mechanism and structural characterization of the CGRP receptor

Author: Kuteyi, G., 6 Dec 2013

Supervisor: Poyner, D. R. (Supervisor) & Bill, R. M. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Mechanisms of ion channel activation in primary cultured and clonal cell lines

Author: Gosling, M., Sep 1995

Supervisor: Poyner, D. R. (Supervisor) & Smith, J. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Reactive oxygen and nitrogen species production in cardiomyoblasts during hypoxia and reoxygenation

Author: Kolamunne, R. K., Apr 2010

Supervisor: Griffiths, H. R. (Supervisor) & Poyner, D. R. (Supervisor)

Student thesis: Doctoral ThesisDoctor of Philosophy

File

Activities 2010 2011

  • 2 Visiting an external academic institution
  • 1 Participation in conference

University of Rochester

David Poyner (Visiting researcher)
2011 → …

Activity: Visiting an external institution typesVisiting an external academic institution

University of Auckland

David Poyner (Visiting researcher)
2011 → …

Activity: Visiting an external institution typesVisiting an external academic institution

7th International Meeting on CGRP

David Poyner (Member of programme committee)
28 Aug 201031 Aug 2011

Activity: Participating in or organising an event typesParticipation in conference