A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice

Bernard Choi; Nikiforos Kollias; Haishan Zeng; Hyun Wook Kang; Brian J. F. Wong; Justus F. Ilgner; Guillermo J. Tearney; Kenton W. Gregory; Laura Marcu; Melissa C. Skala; Paul J. Campagnola; Andreas Mandelis; Michael D. Morris; Karina S. Litvinova; Shakil Ahmad; Keqing Wang; Ilya E. Rafailov; Sergei G. Sokolovski; Lin Zhang; Edik U. Rafailov; Asif Ahmed

doi:10.1117/12.2213026

A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice

Bernard Choi (Editor), Nikiforos Kollias (Editor), Haishan Zeng (Editor), Hyun Wook Kang (Editor), Brian J. F. Wong (Editor), Justus F. Ilgner (Editor), Guillermo J. Tearney (Editor), Kenton W. Gregory (Editor), Laura Marcu (Editor), Melissa C. Skala (Editor), Paul J. Campagnola (Editor), Andreas Mandelis (Editor), Michael D. Morris (Editor), Karina S. Litvinova, Shakil Ahmad, Keqing Wang, Ilya E. Rafailov, Sergei G. Sokolovski, Lin Zhang, Edik U. RafailovAsif Ahmed

Research output: Contribution to conference › Other

Abstract

Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.

Original language	English
DOIs	https://doi.org/10.1117/12.2213026
Publication status	Published - 29 Feb 2016
Event	SPIE BiOS, 2016, San Francisco, California, United States - Duration: 29 Feb 2016 → …

Conference

Conference	SPIE BiOS, 2016, San Francisco, California, United States
Period	29/02/16 → …

Bibliographical note

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Choi, B. (Ed.), Kollias, N. (Ed.), Zeng, H. (Ed.), Kang, H. W. (Ed.), Wong, B. J. F. (Ed.), Ilgner, J. F. (Ed.), Tearney, G. J. (Ed.), Gregory, K. W. (Ed.), Marcu, L. (Ed.), Skala, M. C. (Ed.), Campagnola, P. J. (Ed.), Mandelis, A. (Ed.), Morris, M. D. (Ed.), Litvinova, K. S., Ahmad, S., Wang, K., Rafailov, I. E., Sokolovski, S. G., Zhang, L., ... Ahmed, A. (2016). A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice. SPIE BiOS, 2016, San Francisco, California, United States, . https://doi.org/10.1117/12.2213026

Choi, Bernard (Editor) ; Kollias, Nikiforos (Editor) ; Zeng, Haishan (Editor) ; Kang, Hyun Wook (Editor) ; Wong, Brian J. F. (Editor) ; Ilgner, Justus F. (Editor) ; Tearney, Guillermo J. (Editor) ; Gregory, Kenton W. (Editor) ; Marcu, Laura (Editor) ; Skala, Melissa C. (Editor) ; Campagnola, Paul J. (Editor) ; Mandelis, Andreas (Editor) ; Morris, Michael D. (Editor) ; Litvinova, Karina S. ; Ahmad, Shakil ; Wang, Keqing ; Rafailov, Ilya E. ; Sokolovski, Sergei G. ; Zhang, Lin ; Rafailov, Edik U. ; Ahmed, Asif. / A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice. SPIE BiOS, 2016, San Francisco, California, United States, .

@conference{1415682e1e0944b097339cb671479b8a,

title = "A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice",

abstract = "Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.",

author = "Bernard Choi and Nikiforos Kollias and Haishan Zeng and Kang, {Hyun Wook} and Wong, {Brian J. F.} and Ilgner, {Justus F.} and Tearney, {Guillermo J.} and Gregory, {Kenton W.} and Laura Marcu and Skala, {Melissa C.} and Campagnola, {Paul J.} and Andreas Mandelis and Morris, {Michael D.} and Litvinova, {Karina S.} and Shakil Ahmad and Keqing Wang and Rafailov, {Ilya E.} and Sokolovski, {Sergei G.} and Lin Zhang and Rafailov, {Edik U.} and Asif Ahmed",

note = "{\textcopyright} (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).; SPIE BiOS, 2016, San Francisco, California, United States ; Conference date: 29-02-2016",

year = "2016",

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Choi, B (ed.), Kollias, N (ed.), Zeng, H (ed.), Kang, HW (ed.), Wong, BJF (ed.), Ilgner, JF (ed.), Tearney, GJ (ed.), Gregory, KW (ed.), Marcu, L (ed.), Skala, MC (ed.), Campagnola, PJ (ed.), Mandelis, A (ed.), Morris, MD (ed.), Litvinova, KS , Ahmad, S , Wang, K, Rafailov, IE, Sokolovski, SG, Zhang, L, Rafailov, EU & Ahmed, A 2016, 'A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice', SPIE BiOS, 2016, San Francisco, California, United States, 29/02/16. https://doi.org/10.1117/12.2213026

A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice. / Choi, Bernard (Editor); Kollias, Nikiforos (Editor); Zeng, Haishan (Editor); Kang, Hyun Wook (Editor); Wong, Brian J. F. (Editor); Ilgner, Justus F. (Editor); Tearney, Guillermo J. (Editor); Gregory, Kenton W. (Editor); Marcu, Laura (Editor); Skala, Melissa C. (Editor); Campagnola, Paul J. (Editor); Mandelis, Andreas (Editor); Morris, Michael D. (Editor); Litvinova, Karina S.; Ahmad, Shakil ; Wang, Keqing; Rafailov, Ilya E.; Sokolovski, Sergei G.; Zhang, Lin; Rafailov, Edik U.; Ahmed, Asif.

2016. SPIE BiOS, 2016, San Francisco, California, United States, .

Research output: Contribution to conference › Other

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T1 - A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice

AU - Litvinova, Karina S.

AU - Ahmad, Shakil

AU - Wang, Keqing

AU - Rafailov, Ilya E.

AU - Sokolovski, Sergei G.

AU - Zhang, Lin

AU - Rafailov, Edik U.

AU - Ahmed, Asif

A2 - Choi, Bernard

A2 - Kollias, Nikiforos

A2 - Zeng, Haishan

A2 - Kang, Hyun Wook

A2 - Wong, Brian J. F.

A2 - Ilgner, Justus F.

A2 - Tearney, Guillermo J.

A2 - Gregory, Kenton W.

A2 - Marcu, Laura

A2 - Skala, Melissa C.

A2 - Campagnola, Paul J.

A2 - Mandelis, Andreas

A2 - Morris, Michael D.

PY - 2016/2/29

Y1 - 2016/2/29

N2 - Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.

AB - Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.

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DO - 10.1117/12.2213026

M3 - Other

T2 - SPIE BiOS, 2016, San Francisco, California, United States

Y2 - 29 February 2016

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