Heterologous Expression of Membrane Proteins in E. coli

Peer Depping; María Monserrat Román Lara; Athanasios Kesidis; Roslyn M Bill; Alice J Rothnie; Douglas F Browning; Alan D Goddard

doi:10.1007/978-1-0716-2368-8_4

Heterologous Expression of Membrane Proteins in E. coli

Peer Depping, María Monserrat Román Lara, Athanasios Kesidis, Roslyn M Bill, Alice J Rothnie, Douglas F Browning, Alan D Goddard

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Abstract

Over the decades, the bacterium Escherichia coli (E. coli) has become the cornerstone of recombinant protein production, used for heterologous synthesis of a variety of membrane proteins. Due to its rapid growth to high densities in cheap media, and its ease of manipulation and handling, E. coli is an excellent host cell for a range of membrane protein targets. Furthermore, its genetic tractability allows for a variety of gene constructs to be screened for optimal expression conditions, resulting in relatively high yields of membrane protein in a short amount of time. Here, we describe the general workflow for the production of membrane proteins in E. coli. The protocols we provide show how the gene of interest is modified, transferred to an expression vector and host, and how membrane protein yields can be optimized and analyzed. The examples we illustrate are well suited for scientists who are starting their journey into the world of membrane protein production.

Original language	English
Title of host publication	Heterologous Expression of Membrane Proteins
Subtitle of host publication	Methods in Molecular Biology
Editors	I. Mus-Veteau
Publisher	Springer
Pages	59-78
Number of pages	20
Volume	2507
ISBN (Electronic)	978-1-0716-2368-8
ISBN (Print)	978-1-0716-2367-1
DOIs	https://doi.org/10.1007/978-1-0716-2368-8_4
Publication status	Published - 1 Jul 2022

Publication series

Name	Methods in Molecular Biology
Volume	2507
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Keywords

Escherichia coli
Integral membrane proteins
Outer membrane proteins
Recombinant protein production

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10.1007/978-1-0716-2368-8_4

Heterologous Expression of Membrane Proteins in E. coli
Copyright © Springer Nature B.V. 2022. The final publication is available at Springer via https://doi.org/10.1007/978-1-0716-2368-8_4
Accepted author manuscript, 0.98 MB

Cite this

Depping, P., Román Lara, M. M., Kesidis, A., Bill, R. M., Rothnie, A. J., Browning, D. F., & Goddard, A. D. (2022). Heterologous Expression of Membrane Proteins in E. coli. In I. Mus-Veteau (Ed.), Heterologous Expression of Membrane Proteins: Methods in Molecular Biology (Vol. 2507, pp. 59-78). (Methods in Molecular Biology; Vol. 2507). Springer. https://doi.org/10.1007/978-1-0716-2368-8_4

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Depping, P, Román Lara, MM, Kesidis, A, Bill, RM , Rothnie, AJ , Browning, DF & Goddard, AD 2022, Heterologous Expression of Membrane Proteins in E. coli. in I Mus-Veteau (ed.), Heterologous Expression of Membrane Proteins: Methods in Molecular Biology. vol. 2507, Methods in Molecular Biology, vol. 2507, Springer, pp. 59-78. https://doi.org/10.1007/978-1-0716-2368-8_4

Heterologous Expression of Membrane Proteins in E. coli. / Depping, Peer; Román Lara, María Monserrat; Kesidis, Athanasios et al.
Heterologous Expression of Membrane Proteins: Methods in Molecular Biology. ed. / I. Mus-Veteau. Vol. 2507 Springer, 2022. p. 59-78 (Methods in Molecular Biology; Vol. 2507).

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

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AU - Román Lara, María Monserrat

AU - Kesidis, Athanasios

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AU - Rothnie, Alice J

AU - Browning, Douglas F

AU - Goddard, Alan D

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ER -

Heterologous Expression of Membrane Proteins in E. coli

Abstract

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Keywords

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