20032020

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Personal profile

Research Interests

My research interests lie in elucidating the mechanistic functional details of transmembrane and membrane-associated proteins, that do important jobs in the cell, both in health and disease. To date I have predominantly focused on membrane transporter proteins of the ABC superfamily (ATP Binding Cassette), and proteins involved in cellular trafficking, but recently I have expanded this to include secondary active transporters, ion channels and GPCRs (G-protein coupled receptors). By fully understanding exactly how a protein works, we can begin to understand how it goes wrong in disease states and how we might be able to target it therapeutically. To carry out this work I am really keen to develop new methods and techniques that enable us to answer important questions about the target protein, and as such have played a major role in developing the SMALP (styrene maleic acid lipid particle) technology for membrane protein extraction, purification, structural and functional characterization. This approach is now taking off around the world and has led to numerous new collaborations both within academia and industry.

Employment

  • Jan 2010 – present: Lecturer in Cell Biology/Biochemistry. Aston University, Birmingham, UK.
  • Sept 2007 – Dec 2009: Post-doctoral researcher, Dept. Biological Sciences, Warwick University, Coventry, UK. “Elucidating the mechanism of Hsc70 mediated clathrin cage disassembly”
  • May 2004 – Aug 2007: Post-doctoral fellow, Cancer Research Institute, Queen’s University, Kingston ON, Canada. Recipient of a CIHR (Canadian Institutes Health Research) fellowship.  "The varied roles of GSH in MRP1 transport".

Qualifications

  • Postgraduate Certificate in Professional Practice in Higher Education (PGCPP) with Distinction. Aston University, Birmingham. 2012-2013
  • DPhil, in Biochemistry. Nuffield Dept. Clinical Lab. Sciences, University of Oxford. 2000-2004. Thesis title: "TM6 of P-gp: Changes in topography during the catalytic cycle"
  • MBiochem honours (2.1), in Biochemistry. Brasenose College, University of Oxford. 1996-2000

Membership of Professional Bodies

  • Fellow of the Higher Education Academy
  • Biochemical Society - Member of the 'Molecular Structure & Function' Research Area committee.
  • The Protein Society
  • British Pharmacological Society
  • CBMNet

Teaching Activity

BSc Biological Sciences/Biomedical Science

BY1BC1 - Biochemistry. Module coordinator.

BY2BT2 - Medical Biotechnology.

BSc Biochemistry

BC1003 - Biomolecules. Module coordinator.

BC1001 - Biochemistry Key Skills.

BC2007 - Protein Structure & Function. Module coordinator.

BC2001 - Analytical Biochemistry.

BC2003 - Biochemistry Key Skills II.

BC2004 - Cell membranes & energy generation.

Responsibilities

Biosciences Placement Tutor

Contact Details

Phone number: +44 (0)121 204 4013 
Email:
 a.rothnie@aston.ac.uk
Room number: MB455 

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Research Output

  • 26 Article
  • 3 Review article
  • 1 Chapter (peer-reviewed)
  • 1 Editorial

A comparison of SMA (styrene maleic acid) and DIBMA (di-isobutylene maleic acid) for membrane protein purification

Gulamhussein, A., Uddin, R., Tighe, B., Poyner, D. & Rothnie, A., 21 Mar 2020, In : BBA -Biomembranes. 1862, 183281.

Research output: Contribution to journalArticle

  • Expression and purification of recombinant G protein-coupled receptors: A review

    Wiseman, D. N., Otchere, A., Patel, J. H., Uddin, R., Pollock, N. L., Routledge, S. J., Rothnie, A. J., Slack, C., Poyner, D. R., Bill, R. M. & Goddard, A. D., 1 Mar 2020, In : Protein Expression and Purification. 167, 105524.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Examining the stability of membrane proteins within SMALPs

    Gulamhussein, A. A., Meah, D., Soja, D. D., Fenner, S., Saidani, Z., Akram, A., Lallie, S., Mathews, A., Painter, C., Liddar, M. K., Mohammed, Z., Ki Chiu, L., Sumar, S. S., Healy, H., Hussain, N., Patel, J. H., Hall, S. C. L., Dafforn, T. R. & Rothnie, A. J., 1 Mar 2019, In : European Polymer Journal. 112, p. 120-125 6 p.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Functional expression of Multidrug Resistance Protein 4 MRP4/ABCC4

    Hardy, D., Bill, R. M., Jawhari, A. & Rothnie, A. J., 1 Dec 2019, In : SLAS Discovery. 24, 10, p. 1000-1008 9 p.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Stabilization of human Multidrug Resistance Protein 4 (MRP4/ABCC4) using novel solubilization agents.

    Hardy, D., Bill, R. M., Rothnie, A. J. & Jawhari, A., 1 Dec 2019, In : SLAS Discovery. 24, 10, p. 1009-1017 9 p.

    Research output: Contribution to journalArticle

    Open Access
    File
  • Thesis

    A proteo-liposome system for the analysis of the intracellular interactome of membrane proteins using amyloid precursor protein as a model

    Author: Currinn, H., 9 Jun 2015

    Supervisor: Wassmer, T. (Supervisor) & Rothnie, A. J. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    ATP-binding cassette subfamily C (ABCC) transporter 1 (ABCC1) and 4 (ABCC4) independent of their drug efflux ability affects breast cancer biology

    Author: Low, F., 15 May 2019

    Supervisor: Rothnie, A. (Supervisor) & Bill, R. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Recombinant synthesis of pulmonary surfactant proteins SP-B and SP-C

    Author: Patel, A., 20 Sep 2018

    Supervisor: Bill, R. M. (Supervisor) & Rothnie, A. J. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Solubilisation and stabilisation of MRP4 using novel detergents for function and structural studies

    Author: Hardy, D., 13 Mar 2019

    Supervisor: Rothnie, A. J. (Supervisor) & Bill, R. M. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Prizes

    CIHR Post-doctoral fellowship

    Alice Rothnie (Recipient), Aug 2006

    Prize: Fellowship awarded competitively

    Activities

    SMALP meeting 2019

    Alice Rothnie (Participant)
    25 Apr 2019

    Activity: Participating in or organising an event typesParticipation in conference

    2nd UK workshop on membrane proteins: solubilisation & biophysical characterisation

    Alice Rothnie (Participant)
    3 Apr 20195 Apr 2019

    Activity: Participating in or organising an event typesOrganising a conference, workshop, seminar or course

    A taste of bioschemistry

    Alice Rothnie (Recipient)
    1 Apr 2019

    Activity: Other activity typesTypes of Public engagement and outreach - Schools engagement

    Experimental techniques for studying proteins and lipids in biological membranes.

    Alice Rothnie (Speaker)
    31 Jul 2018

    Activity: Talk or presentation typesInvited talk

    ABC2018 - 7th FEBS Special MeetingATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases

    Alice Rothnie (Participant)
    6 Mar 201812 Mar 2018

    Activity: Participating in or organising an event typesParticipation in conference