Intracellular redox state modulates the T cell surface proteome – relevance to ageing

Stuart J. Bennett, Chris R. Dunston, Irundika H.K. Dias, Rita Carilho, Edyta Augustyniak, Helen R. Griffiths

Research output: Contribution to journalConference abstract

Abstract

During ageing an altered redox balance has been observed in both intracellular and extracellular compartments, primarily due to glutathione depletion and metabolic stress. Maintaining redox homeostasis is important for controlling proliferation and apoptosis in response to specific stimuli for a variety of cells. For T cells, the ability to generate specific response to antigen is dependent on the oxidation state of cell surface and cytoplasmic protein-thiols. Here we describe the effects of depleting intracellular glutathione concentration for T cell exofacial expression of thioredoxin 1 and IL-2 production, and have determined the distribution of Trx1 with ageing. Using buthionine sulfoximine to deplete intracellular glutathione in Jurkat T cells we show using Western blotting that cell surface thioredoxin-1 is lowered and that the response to the lectin phytohaemagglutinin measured by ELISA as IL-2 production is also decreased. Using flow cytometry we show that the distribution of Trx1 on primary CD4+ T cells is age-dependent, with lower surface Trx1 expression and greater variability of surface expression observed with age. Together these data suggest that a relationship exists between the intracellular redox compartment and exofacial surface. Redox imbalance may be important for impaired T cell function during ageing.
Original languageEnglish
Pages (from-to)S11
Number of pages1
JournalFree Radical Biology and Medicine
Volume65
Issue numberSuppl.1
DOIs
Publication statusPublished - 20 Sep 2013
EventSFRR - Europe 2013 meeting - Athens, Greece
Duration: 23 Sep 201325 Sep 2013

Bibliographical note

SFRR - Europe 2013 Meeting "The new era of -omics in Free Radicals in Biology and Medicine", 23 - 25 Sep 2013, Athens, Greece.

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    • 4 Meeting abstract

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