Intracellular redox state modulates the T cell surface proteome – relevance to ageing

Stuart J. Bennett, Chris R. Dunston, Irundika H.K. Dias, Rita Carilho, Edyta Augustyniak, Helen R. Griffiths

Research output: Contribution to journalMeeting abstract

Abstract

During ageing an altered redox balance has been observed in both intracellular and extracellular compartments, primarily due to glutathione depletion and metabolic stress. Maintaining redox homeostasis is important for controlling proliferation and apoptosis in response to specific stimuli for a variety of cells. For T cells, the ability to generate specific response to antigen is dependent on the oxidation state of cell surface and cytoplasmic protein-thiols. Here we describe the effects of depleting intracellular glutathione concentration for T cell exofacial expression of thioredoxin 1 and IL-2 production, and have determined the distribution of Trx1 with ageing. Using buthionine sulfoximine to deplete intracellular glutathione in Jurkat T cells we show using Western blotting that cell surface thioredoxin-1 is lowered and that the response to the lectin phytohaemagglutinin measured by ELISA as IL-2 production is also decreased. Using flow cytometry we show that the distribution of Trx1 on primary CD4+ T cells is age-dependent, with lower surface Trx1 expression and greater variability of surface expression observed with age. Together these data suggest that a relationship exists between the intracellular redox compartment and exofacial surface. Redox imbalance may be important for impaired T cell function during ageing.
Original languageEnglish
Pages (from-to)S11
Number of pages1
JournalFree Radical Biology and Medicine
Volume65
Issue numberSuppl.1
DOIs
Publication statusPublished - 20 Sep 2013
EventSFRR - Europe 2013 meeting - Athens, Greece
Duration: 23 Sep 201325 Sep 2013

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T-cells
Proteome
Oxidation-Reduction
Aging of materials
T-Lymphocytes
Glutathione
Thioredoxins
Interleukin-2
Buthionine Sulfoximine
Physiological Stress
Jurkat Cells
Flow cytometry
Phytohemagglutinins
Sulfhydryl Compounds
Lectins
Flow Cytometry
Membrane Proteins
Homeostasis
Western Blotting
Enzyme-Linked Immunosorbent Assay

Bibliographical note

SFRR - Europe 2013 Meeting "The new era of -omics in Free Radicals in Biology and Medicine", 23 - 25 Sep 2013, Athens, Greece.

Cite this

Bennett, Stuart J. ; Dunston, Chris R. ; Dias, Irundika H.K. ; Carilho, Rita ; Augustyniak, Edyta ; Griffiths, Helen R. / Intracellular redox state modulates the T cell surface proteome – relevance to ageing. In: Free Radical Biology and Medicine. 2013 ; Vol. 65, No. Suppl.1. pp. S11.
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abstract = "During ageing an altered redox balance has been observed in both intracellular and extracellular compartments, primarily due to glutathione depletion and metabolic stress. Maintaining redox homeostasis is important for controlling proliferation and apoptosis in response to specific stimuli for a variety of cells. For T cells, the ability to generate specific response to antigen is dependent on the oxidation state of cell surface and cytoplasmic protein-thiols. Here we describe the effects of depleting intracellular glutathione concentration for T cell exofacial expression of thioredoxin 1 and IL-2 production, and have determined the distribution of Trx1 with ageing. Using buthionine sulfoximine to deplete intracellular glutathione in Jurkat T cells we show using Western blotting that cell surface thioredoxin-1 is lowered and that the response to the lectin phytohaemagglutinin measured by ELISA as IL-2 production is also decreased. Using flow cytometry we show that the distribution of Trx1 on primary CD4+ T cells is age-dependent, with lower surface Trx1 expression and greater variability of surface expression observed with age. Together these data suggest that a relationship exists between the intracellular redox compartment and exofacial surface. Redox imbalance may be important for impaired T cell function during ageing.",
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Intracellular redox state modulates the T cell surface proteome – relevance to ageing. / Bennett, Stuart J.; Dunston, Chris R.; Dias, Irundika H.K.; Carilho, Rita; Augustyniak, Edyta; Griffiths, Helen R.

In: Free Radical Biology and Medicine, Vol. 65, No. Suppl.1, 20.09.2013, p. S11.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Intracellular redox state modulates the T cell surface proteome – relevance to ageing

AU - Bennett, Stuart J.

AU - Dunston, Chris R.

AU - Dias, Irundika H.K.

AU - Carilho, Rita

AU - Augustyniak, Edyta

AU - Griffiths, Helen R.

N1 - SFRR - Europe 2013 Meeting "The new era of -omics in Free Radicals in Biology and Medicine", 23 - 25 Sep 2013, Athens, Greece.

PY - 2013/9/20

Y1 - 2013/9/20

N2 - During ageing an altered redox balance has been observed in both intracellular and extracellular compartments, primarily due to glutathione depletion and metabolic stress. Maintaining redox homeostasis is important for controlling proliferation and apoptosis in response to specific stimuli for a variety of cells. For T cells, the ability to generate specific response to antigen is dependent on the oxidation state of cell surface and cytoplasmic protein-thiols. Here we describe the effects of depleting intracellular glutathione concentration for T cell exofacial expression of thioredoxin 1 and IL-2 production, and have determined the distribution of Trx1 with ageing. Using buthionine sulfoximine to deplete intracellular glutathione in Jurkat T cells we show using Western blotting that cell surface thioredoxin-1 is lowered and that the response to the lectin phytohaemagglutinin measured by ELISA as IL-2 production is also decreased. Using flow cytometry we show that the distribution of Trx1 on primary CD4+ T cells is age-dependent, with lower surface Trx1 expression and greater variability of surface expression observed with age. Together these data suggest that a relationship exists between the intracellular redox compartment and exofacial surface. Redox imbalance may be important for impaired T cell function during ageing.

AB - During ageing an altered redox balance has been observed in both intracellular and extracellular compartments, primarily due to glutathione depletion and metabolic stress. Maintaining redox homeostasis is important for controlling proliferation and apoptosis in response to specific stimuli for a variety of cells. For T cells, the ability to generate specific response to antigen is dependent on the oxidation state of cell surface and cytoplasmic protein-thiols. Here we describe the effects of depleting intracellular glutathione concentration for T cell exofacial expression of thioredoxin 1 and IL-2 production, and have determined the distribution of Trx1 with ageing. Using buthionine sulfoximine to deplete intracellular glutathione in Jurkat T cells we show using Western blotting that cell surface thioredoxin-1 is lowered and that the response to the lectin phytohaemagglutinin measured by ELISA as IL-2 production is also decreased. Using flow cytometry we show that the distribution of Trx1 on primary CD4+ T cells is age-dependent, with lower surface Trx1 expression and greater variability of surface expression observed with age. Together these data suggest that a relationship exists between the intracellular redox compartment and exofacial surface. Redox imbalance may be important for impaired T cell function during ageing.

U2 - 10.1016/j.freeradbiomed.2013.08.129

DO - 10.1016/j.freeradbiomed.2013.08.129

M3 - Meeting abstract

VL - 65

SP - S11

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - Suppl.1

ER -