@inbook{ef4830cbb84f43f58d95bd22d40d21cb,
title = "Membrane Protein Production in Insect Cells",
abstract = "Membrane proteins are an essential part of the machinery of life. They connect the interior and exterior of cells, play an important role in cell signaling and are responsible for the influx and efflux of nutrients and metabolites. For their structural and functional analysis high yields of correctly folded and modified protein are needed. Insect cells, such as Sf9 cells, have been one of the major expression hosts for eukaryotic membrane proteins in structural investigations during the last decade, as they are easier to handle than mammalian cells and provide more natural posttranslational modifications than microbial systems. Here we describe general techniques for establishing and maintaining insect cell cultures, the generation and amplification of recombinant baculovirus stocks using the flashBAC{\texttrademark} or Bac-to-Bac{\texttrademark} systems, membrane protein production, as well as the production of membrane preparations for extraction and purification experiments.",
keywords = "Animals, Baculoviridae/genetics, Genetic Vectors, Insecta/metabolism, Mammals/metabolism, Membrane Proteins/genetics, Protein Processing, Post-Translational, Recombinant Proteins/genetics, Sf9 Cells, Spodoptera/metabolism, Recombinant baculovirus, Sf9 insect cells, Membrane protein production",
author = "Afroditi Vaitsopoulou and Peer Depping and Bill, {Roslyn M} and Goddard, {Alan D} and Rothnie, {Alice J}",
note = "Copyright {\textcopyright} Springer Nature B.V. 2022. The final publication is available at Springer via https://doi.org/10.1007/978-1-0716-2368-8_12",
year = "2022",
month = jul,
day = "1",
doi = "10.1007/978-1-0716-2368-8_12",
language = "English",
isbn = "978-1-0716-2367-1",
volume = "2507",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "223--240",
editor = "I. Mus-Veteau",
booktitle = "Methods in Molecular Biology",
address = "Germany",
}