A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice

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Authors

  • Bernard Choi (Editor)
  • Nikiforos Kollias (Editor)
  • Haishan Zeng (Editor)
  • Hyun Wook Kang (Editor)
  • Brian J. F. Wong (Editor)
  • Justus F. Ilgner (Editor)
  • Guillermo J. Tearney (Editor)
  • Kenton W. Gregory (Editor)
  • Laura Marcu (Editor)
  • Melissa C. Skala (Editor)
  • Paul J. Campagnola (Editor)
  • Andreas Mandelis (Editor)
  • Michael D. Morris (Editor)
  • Ilya E. Rafailov
  • Sergei G. Sokolovski

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Abstract

Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.

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Original languageEnglish
DOIs
Publication statusPublished - 29 Feb 2016
EventSPIE BiOS, 2016, San Francisco, California, United States -
Duration: 29 Feb 2016 → …

Conference

ConferenceSPIE BiOS, 2016, San Francisco, California, United States
Period29/02/16 → …

Bibliographic note

© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

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