S100P enhances the motility and invasion of human trophoblast cell lines

Maral Ebrahimzadeh Asl Tabrizi, Tara Lancaster, Thamir M. Ismail, Ankana Ganguly, Athina Georgiadou, Keqing Wang, Philip S. Rudland, Shakil S Ahmad, Stephane R Gross

Research output: Contribution to journalArticle

Abstract

S100P has been shown to be a marker for carcinogenesis where its expression in solid tumours correlates with metastasis and a poor patient prognosis. This protein’s role in any physiological process is, however, unknown. Here we first show that S100P is expressed both in trophoblasts in vivo as well as in some corresponding cell lines in culture. We demonstrate that S100P is predominantly expressed during the early stage of placental formation with its highest expression levels occurring during the first trimester of gestation, particularly in the invading columns and anchoring villi. Using gain or loss of function studies through overexpression or knockdown of S100P expression respectively, our work shows that S100P stimulates both cell motility and cellular invasion in different trophoblastic and first trimester EVT cell lines. Interestingly, cell invasion was seen to be more dramatically affected than cell migration. Our results suggest that S100P may be acting as an important regulator of trophoblast invasion during placentation. This finding sheds new light on a hitherto uncharacterized molecular mechanism which may, in turn, lead to the identification of novel targets that may explain why significant numbers of confirmed human pregnancies suffer complications through poor placental implantation.
LanguageEnglish
Article number11488
JournalScientific Reports
Volume8
DOIs
Publication statusPublished - 31 Jul 2018

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Trophoblasts
First Pregnancy Trimester
Cell Movement
Physiological Phenomena
Cell Line
Placentation
Pregnancy Complications
Carcinogenesis
Neoplasm Metastasis
Pregnancy
Neoplasms
Proteins

Bibliographical note

This article is licensed under a Creative Commons Attribution 4.0 International
License, which permits use, sharing, adaptation, distribution and reproduction in any medium or
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Commons license, and indicate if changes were made. Te images or other third party material in this
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© The Author(s) 2018

Cite this

Ebrahimzadeh Asl Tabrizi, M., Lancaster, T., Ismail, T. M., Ganguly, A., Georgiadou, A., Wang, K., ... Gross, S. R. (2018). S100P enhances the motility and invasion of human trophoblast cell lines. Scientific Reports, 8, [11488]. https://doi.org/10.1038/s41598-018-29852-2
Ebrahimzadeh Asl Tabrizi, Maral ; Lancaster, Tara ; Ismail, Thamir M. ; Ganguly, Ankana ; Georgiadou, Athina ; Wang, Keqing ; Rudland, Philip S. ; Ahmad, Shakil S ; Gross, Stephane R. / S100P enhances the motility and invasion of human trophoblast cell lines. In: Scientific Reports. 2018 ; Vol. 8.
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Ebrahimzadeh Asl Tabrizi, M, Lancaster, T, Ismail, TM, Ganguly, A, Georgiadou, A, Wang, K, Rudland, PS, Ahmad, SS & Gross, SR 2018, 'S100P enhances the motility and invasion of human trophoblast cell lines' Scientific Reports, vol. 8, 11488. https://doi.org/10.1038/s41598-018-29852-2

S100P enhances the motility and invasion of human trophoblast cell lines. / Ebrahimzadeh Asl Tabrizi, Maral; Lancaster, Tara; Ismail, Thamir M.; Ganguly, Ankana; Georgiadou, Athina; Wang, Keqing; Rudland, Philip S.; Ahmad, Shakil S; Gross, Stephane R.

In: Scientific Reports, Vol. 8, 11488, 31.07.2018.

Research output: Contribution to journalArticle

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Ebrahimzadeh Asl Tabrizi M, Lancaster T, Ismail TM, Ganguly A, Georgiadou A, Wang K et al. S100P enhances the motility and invasion of human trophoblast cell lines. Scientific Reports. 2018 Jul 31;8. 11488. https://doi.org/10.1038/s41598-018-29852-2