The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review)

Nagamani Bora, Zharain Bawa, Roslyn M. Bill, Martin D.B. Wilks

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Biological processes are subject to the influence of numerous factors and their interactions, which may be non-linear in nature. In a recombinant protein production experiment, understanding the relative importance of these factors, and their influence on the yield and quality of the recombinant protein being produced, is an essential part of its optimisation. In many cases, implementing a design of experiments (DoE) approach has delivered this understanding. This chapter aims to provide the reader with useful pointers in applying a DoE strategy to improve the yields of recombinant yeast cultures.
Original languageEnglish
Title of host publicationRecombinant protein production in yeast
Subtitle of host publicationmethods and protocols
EditorsRoslyn M. Bill
PublisherHumana Press
Pages115-127
Number of pages13
ISBN (Electronic)978-1-61779-770-5
ISBN (Print)978-1-61779-769-9
DOIs
Publication statusPublished - 23 Apr 2012

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume866
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029
NameSpringer protocols
PublisherSpringer

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  • Research Output

    • 6 Chapter (peer-reviewed)
    • 1 Anthology
    • 1 Foreword/postscript

    Large-scale production of secreted proteins in Pichia pastoris

    Bora, N., 25 Feb 2012, Recombinant protein production in yeast. Bill, R. M. (ed.). Humana Press, p. 217-235 19 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Optimising Pichia pastoris induction

    Bawa, Z. & Darby, R. A. J., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 181-190 10 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Optimising yeast as a host for recombinant protein production (review)

    Bonander, N. & Bill, R. M., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 1-9 9 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

  • Cite this

    Bora, N., Bawa, Z., Bill, R. M., & Wilks, M. D. B. (2012). The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review). In R. M. Bill (Ed.), Recombinant protein production in yeast: methods and protocols (pp. 115-127). (Methods in molecular biology; Vol. 866), (Springer protocols). Humana Press. https://doi.org/10.1007/978-1-61779-770-5_11