The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review)

Nagamani Bora; Zharain Bawa; Roslyn M. Bill; Martin D.B. Wilks

doi:10.1007/978-1-61779-770-5_11

The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review)

Nagamani Bora, Zharain Bawa, Roslyn M. Bill, Martin D.B. Wilks

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Abstract

Biological processes are subject to the influence of numerous factors and their interactions, which may be non-linear in nature. In a recombinant protein production experiment, understanding the relative importance of these factors, and their influence on the yield and quality of the recombinant protein being produced, is an essential part of its optimisation. In many cases, implementing a design of experiments (DoE) approach has delivered this understanding. This chapter aims to provide the reader with useful pointers in applying a DoE strategy to improve the yields of recombinant yeast cultures.

Original language	English
Title of host publication	Recombinant protein production in yeast
Subtitle of host publication	methods and protocols
Editors	Roslyn M. Bill
Publisher	Humana Press
Pages	115-127
Number of pages	13
ISBN (Electronic)	978-1-61779-770-5
ISBN (Print)	978-1-61779-769-9
DOIs	https://doi.org/10.1007/978-1-61779-770-5_11
Publication status	Published - 23 Apr 2012

Publication series

Name	Methods in molecular biology
Publisher	Humana Press
Volume	866
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Name	Springer protocols
Publisher	Springer

Access to Document

10.1007/978-1-61779-770-5_11

Cite this

Bora, N., Bawa, Z., Bill, R. M., & Wilks, M. D. B. (2012). The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review). In R. M. Bill (Ed.), Recombinant protein production in yeast: methods and protocols (pp. 115-127). (Methods in molecular biology; Vol. 866), (Springer protocols). Humana Press. https://doi.org/10.1007/978-1-61779-770-5_11

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Bora, N, Bawa, Z, Bill, RM & Wilks, MDB 2012, The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review). in RM Bill (ed.), Recombinant protein production in yeast: methods and protocols. Methods in molecular biology, vol. 866, Springer protocols, Humana Press, pp. 115-127. https://doi.org/10.1007/978-1-61779-770-5_11

The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review). / Bora, Nagamani; Bawa, Zharain; Bill, Roslyn M. et al.
Recombinant protein production in yeast: methods and protocols. ed. / Roslyn M. Bill. Humana Press, 2012. p. 115-127 (Methods in molecular biology; Vol. 866), (Springer protocols).

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

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BT - Recombinant protein production in yeast

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The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review)

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