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• 2019

  Interactions Between RAMP2 And CRF Receptors: The Effect Of Receptor Subtypes, Splice Variants And Cell Context

  Bailey, S., Harris, M., Barkan, K., Winfield, I., Thomas-Harper, M., Simms, J. W., Ladds, G., Wheatley, M. & Poyner, D. R., 1 May 2019, In: BBA -Biomembranes. 1861, 5, p. 997-1003 7 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Corticotropin-Releasing Hormone Receptors
  • Corticotropin-Releasing Hormone
  • Calcitonin Receptors
  • Cell Line
  • Histidine
• 2018

  Functional characterisation of G protein-coupled receptors

  Uddin, R., Simms, J. & Poyner, D., 1 Sep 2018, In: Methods. 147, p. 213-220

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Forster resonance energy transfer
  • G-Protein-Coupled Receptors
  • Ligands
  • Fluorescence Resonance Energy Transfer
  • GTP-Binding Proteins

• hCALCRL mutation causes autosomal recessive nonimmune hydrops fetalis with lymphatic dysplasia

  Mackie, D. I., Al Mutairi, F., Davis, R. B., Kechele, D. O., Nielsen, N. R., Snyder, J. C., Caron, M. G., Kliman, H. J., Berg, J. S., Simms, J., Poyner, D. R. & Caron, K. M., 16 Aug 2018, In: Journal of Experimental Medicine . 215, 9, p. 2339-2353

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Hydrops Fetalis
  • Mutation
  • Receptor Activity-Modifying Proteins
  • Calcitonin Receptor-Like Protein
  • Fetal Death

• Microbial expression systems for membrane proteins

  Dilworth, M. V., Piel, M. S., Bettaney, K. E., Ma, P., Luo, J., Sharples, D., Poyner, D. R., Gross, S. R., Moncoq, K., J. F. Henderson, P., Miroux, B. & Bill, R. M., 12 Apr 2018, In: Methods.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Membrane Proteins
  • Genetic engineering
  • Cell culture
  • Catalogs
  • Recombinant DNA

• Photoaffinity cross-linking and unnatural amino acid mutagenesis reveal insights into calcitonin gene-related peptide binding to the calcitonin receptor-like receptor/receptor activity-modifying protein 1 (CLR/RAMP1) complex

  Simms, J., Uddin, R., Sakmar, T. P., Gingell, J. J., Garelja, M. L., Hay, D. L., Brimble, M. A., Harris, P. W. R., Reynolds, C. A. & Poyner, D. R., 14 Aug 2018, In: Biochemistry. 57, 32, p. 4915-4922

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Receptor Activity-Modifying Protein 1
  • Calcitonin Receptor-Like Protein
  • Mutagenesis
  • Calcitonin Gene-Related Peptide
  • Amino Acids

• The structure of the CGRP and related receptors

  Simms, J., Routledge, S., Uddin, R. & Poyner, D., 25 May 2018, Handbook of Experimental Pharmacology. Brain, S. & Gepetti, P. (eds.). Springer, p. 23-36 14 p. (Handbook of Experimental Pharmacology; vol. 255).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

  • Calcitonin Receptor-Like Protein
  • Calcitonin Gene-Related Peptide Receptors
  • Receptor Activity-Modifying Protein 1
  • Calcitonin Receptors
  • Crystal structure
• 2017

  Receptor activity-modifying protein dependent and independent activation mechanisms in the coupling of calcitonin gene-related peptide and adrenomedullin receptors to Gs

  Woolley, M. J., Reynolds, C. A., Simms, J., Walker, C. S., Mobarec, J. C., Garelja, M. L., Conner, A. C., Poyner, D. R. & Hay, D. L., 15 Oct 2017, In: Biochemical Pharmacology. 142, p. 96-110

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Receptor Activity-Modifying Proteins
  • Calcitonin Receptor-Like Protein
  • Adrenomedullin Receptors
  • Calcitonin Gene-Related Peptide Receptors
  • Chemical activation

• Relative antagonism of mutants of the CGRP receptor extracellular loop 2 domain (ECL2) using a truncated competitive antagonist (CGRP_8-37): evidence for the dual involvement of ECL2 in the two-domain binding model

  Woolley, M. J., Simms, J., Uddin, S., Poyner, D. R. & Conner, A. C., Aug 2017, In: Biochemistry. 56, 30, p. 3877-3880 4 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • calcitonin gene-related peptide (8-37)
  • Calcitonin Gene-Related Peptide Receptors
  • G-Protein-Coupled Receptors
  • Chemical activation
  • CCR10 Receptors

• The effects of RAMPs upon cell signalling

  Routledge, S. J., Ladds, G. & Poyner, D. R., 5 Apr 2017, In: Molecular and Cellular Endocrinology. in press

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Receptor Activity-Modifying Proteins
  • Cell signaling
  • G-Protein-Coupled Receptors
  • Signal transduction
  • GTP-Binding Proteins

• Understanding the molecular functions of the second extracellular loop (ECL2) of the calcitonin gene-related peptide (CGRP) receptor using a comprehensive mutagenesis approach

  Woolley, M. J., Simms, J., Mobarec, J. C., Reynolds, C. A., Poyner, D. R. & Conner, A. C., 15 Oct 2017, In: Molecular and Cellular Endocrinology. 454, p. 39-49

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Mutagenesis
  • Calcitonin Gene-Related Peptide Receptors
  • G-Protein-Coupled Receptors
  • Substitution reactions
  • Ligands

• Update on the pharmacology of calcitonin/CGRP family of peptides: IUPHAR Review 25

  Hay, D. L., Garelja, M. L., Poyner, D. R. & Walker, C. S., 28 Nov 2017, In: British Journal of Pharmacology. In press, 41 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Calcitonin Receptors
  • Calcitonin Gene-Related Peptide
  • Calcitonin
  • Calcitonin Receptor-Like Protein
  • Calcitonin Gene-Related Peptide Receptors
• 2016

  An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology

  Gingell, J. J., Simms, J., Barwell, J., Poyner, D. R., Watkins, H. A., Pioszak, AA., Sexton, P. M. & Hay, D. L., 17 May 2016, In: Cell Discovery. 2, 16012.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Receptor Activity-Modifying Proteins
  • Calcitonin Receptors
  • Pharmacology
  • G-Protein-Coupled Receptors
  • Ligands

• GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential

  Wheatley, M., Charlton, J., Jamshad, M., Routledge, S. J., Bailey, S., La-Borde, P. J., Azam, M. T., Logan, R. T., Bill, R. M., Dafforn, T. R. & Poyner, D. R., 15 Apr 2016, In: Biochemical Society Transactions. 44, 2, p. 619-623 5 p.

  Research output: Contribution to journal › Review article › peer-review

  Open Access

  File

  • maleic acid
  • Styrene
  • Detergents
  • G-Protein-Coupled Receptors
  • styrene-maleic acid polymer

• Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors

  Weston, C., Winfield, I., Harris, M., Hodgson, R., Shah, A., Dowell, S. J., Mobarec, J. C., Woodlock, D. A., Reynolds, C. A., Poyner, D. R., Watkins, H. A. & Ladds, G., 14 Oct 2016, In: Journal of Biological Chemistry. 291, 42, p. 21925-21944 20 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Receptor Activity-Modifying Proteins
  • Calcitonin Gene-Related Peptide Receptors
  • Calcitonin Gene-Related Peptide
  • GTP-Binding Proteins
  • Calcitonin Receptor-Like Protein

• Receptor activity-modifying proteins; multifunctional G protein-coupled receptor accessory proteins

  Hay, D. L., Walker, C. S., Gingell, J. J., Ladds, G., Reynolds, C. A. & Poyner, D. R., 15 Apr 2016, In: Biochemical Society Transactions. 44, 2, p. 568-573 6 p.

  Research output: Contribution to journal › Review article › peer-review

  • Receptor Activity-Modifying Proteins
  • G-Protein-Coupled Receptors
  • Calcitonin Receptor-Like Protein
  • Proteins
  • Drug Delivery Systems

• Receptor activity-modifying proteins 2 and 3 generate adrenomedullin receptor subtypes with distinct molecular properties

  Watkins, H. A., Chakravarthy, M., Abhayawardana, R. S., Gingell, J. J., Garelja, M., Pardamwar, M., McElhinney, J. M. W. R., Lathbridge, A., Constantine, A., Harris, P. W. R., Yuen, T-Y., Brimble, M. A., Barwell, J., Poyner, D. R., Woolley, M. J., Conner, A. C., Pioszak, AA., Reynolds, C. A. & Hay, D. L., 27 May 2016, In: Journal of Biological Chemistry. 291, 22, p. 11657-11675 19 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Receptor Activity-Modifying Protein 3
  • Receptor Activity-Modifying Protein 2
  • Calcitonin Receptor-Like Protein
  • Adrenomedullin Receptors
  • Adrenomedullin
• 2015

  G-protein-coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent

  Jamshad, M., Charlton, J., Lin, Y-P., Routledge, S. J., Bawa, Z., Knowles, T. J., Overduin, M., Dekker, N., Dafforn, T. R., Bill, R. M., Poyner, D. R. & Wheatley, M., 27 Feb 2015, In: Bioscience Reports. 35, 2, 10 p., e00188.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Purification
  • Detergents
  • G-Protein-Coupled Receptors
  • styrene-maleic acid polymer
  • Lipids

• Modulation of glucagon receptor pharmacology by Receptor Activity-modifying Protein-2 (RAMP2)

  Weston, C., Lu, J., Li, N., Barkan, K., Richards, G. O., Roberts, D. J., Skerry, T. M., Poyner, D., Pardamwar, M., Reynolds, C. A., Dowell, S. J., Willars, G. B. & Ladds, G., 18 Sep 2015, In: Journal of Biological Chemistry. 290, 38, p. 23009-23022 14 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Receptor Activity-Modifying Protein 2
  • Glucagon Receptors
  • Glucagon-Like Peptide-1 Receptor
  • Modulation
  • Pharmacology

• Structural basis for receptor activity-modifying protein-dependent selective peptide recognition by a G protein-coupled receptor

  Booe, JM., Walker, C. S., Barwell, J., Kuteyi, G., Simms, J., Jamaluddin, MA., Warner, ML., Bill, R. M., Harris, PW., Brimble, MA., Poyner, D. R., Hay, D. L. & Pioszak, AA., 18 Jun 2015, In: Molecular Cell. 58, 6, p. 1040-1052 13 p.

  Research output: Contribution to journal › Article › peer-review

  Open Access

  File

  • Receptor Activity-Modifying Proteins
  • Calcitonin Receptor-Like Protein
  • G-Protein-Coupled Receptors
  • Peptides
  • Adrenomedullin