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2019

Interactions Between RAMP2 And CRF Receptors: The Effect Of Receptor Subtypes, Splice Variants And Cell Context

Bailey, S., Harris, M., Barkan, K., Winfield, I., Thomas-Harper, M., Simms, J. W., Ladds, G., Wheatley, M. & Poyner, D. R., 1 May 2019, In : BBA -Biomembranes. 1861, 5, p. 997-1003 7 p.

Research output: Contribution to journalArticle

Corticotropin-Releasing Hormone Receptors
Corticotropin-Releasing Hormone
Calcitonin Receptors
Cell Line
Histidine
2018

Functional characterisation of G protein-coupled receptors

Uddin, R., Simms, J. & Poyner, D., 3 Mar 2018, In : Methods.

Research output: Contribution to journalArticle

Open Access
File
G-Protein-Coupled Receptors
Ligands
Fluorescence Resonance Energy Transfer
GTP-Binding Proteins
Chemical activation

hCALCRL mutation causes autosomal recessive nonimmune hydrops fetalis with lymphatic dysplasia

Mackie, D. I., Al Mutairi, F., Davis, R. B., Kechele, D. O., Nielsen, N. R., Snyder, J. C., Caron, M. G., Kliman, H. J., Berg, J. S., Simms, J., Poyner, D. R. & Caron, K. M., 16 Aug 2018, In : Journal of Experimental Medicine . 215, 9, p. 2339-2353

Research output: Contribution to journalArticle

Open Access
File
Hydrops Fetalis
Mutation
Receptor Activity-Modifying Proteins
Calcitonin Receptor-Like Protein
Fetal Death

Microbial expression systems for membrane proteins

Dilworth, M. V., Piel, M. S., Bettaney, K. E., Ma, P., Luo, J., Sharples, D., Poyner, D. R., Gross, S. R., Moncoq, K., J. F. Henderson, P., Miroux, B. & Bill, R. M., 12 Apr 2018, In : Methods.

Research output: Contribution to journalArticle

Open Access
File
Membrane Proteins
Genetic engineering
Proteins
Recombinant DNA
Crystallization

Photoaffinity cross-linking and unnatural amino acid mutagenesis reveal insights into calcitonin gene-related peptide binding to the calcitonin receptor-like receptor/receptor activity-modifying protein 1 (CLR/RAMP1) complex

Simms, J., Uddin, R., Sakmar, T. P., Gingell, J. J., Garelja, M. L., Hay, D. L., Brimble, M. A., Harris, P. W. R., Reynolds, C. A. & Poyner, D. R., 13 Jul 2018, In : Biochemistry.

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Protein 1
Calcitonin Receptor-Like Protein
Mutagenesis
Calcitonin Gene-Related Peptide
Amino Acids

The structure of the CGRP and related receptors

Simms, J., Routledge, S., Uddin, R. & Poyner, D., 25 May 2018, Handbook of Experimental Pharmacology. Brain, S. & Gepetti, P. (eds.). Springer, p. 23-36 14 p. (Handbook of Experimental Pharmacology; vol. 255).

Research output: Chapter in Book/Report/Conference proceedingChapter

Calcitonin Receptor-Like Protein
Calcitonin Gene-Related Peptide Receptors
Receptor Activity-Modifying Protein 1
Calcitonin Receptors
G-Protein-Coupled Receptors
2017

Receptor activity-modifying protein dependent and independent activation mechanisms in the coupling of calcitonin gene-related peptide and adrenomedullin receptors to Gs

Woolley, M. J., Reynolds, C. A., Simms, J., Walker, C. S., Mobarec, J. C., Garelja, M. L., Conner, A. C., Poyner, D. R. & Hay, D. L., 11 Jul 2017, In : Biochemical Pharmacology. in press

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Proteins
Calcitonin Receptor-Like Protein
Adrenomedullin Receptors
Calcitonin Gene-Related Peptide Receptors
Adrenomedullin
Open Access
File
Calcitonin Gene-Related Peptide Receptors
G-Protein-Coupled Receptors
CCR10 Receptors
Chemical activation
Cell signaling

The effects of RAMPs upon cell signalling

Routledge, S. J., Ladds, G. & Poyner, D. R., 5 Apr 2017, In : Molecular and Cellular Endocrinology. in press

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Proteins
Cell signaling
G-Protein-Coupled Receptors
Signal transduction
GTP-Binding Proteins

Understanding the molecular functions of the second extracellular loop (ECL2) of the calcitonin gene-related peptide (CGRP) receptor using a comprehensive mutagenesis approach

Woolley, M. J., Simms, J., Mobarec, J. C., Reynolds, C. A., Poyner, D. R. & Conner, A. C., 30 May 2017, In : Molecular and Cellular Endocrinology. in press

Research output: Contribution to journalArticle

Open Access
File
Calcitonin Gene-Related Peptide Receptors
Mutagenesis
G-Protein-Coupled Receptors
Substitution reactions
Ligands

Update on the pharmacology of calcitonin/CGRP family of peptides: IUPHAR Review 25

Hay, D. L., Garelja, M. L., Poyner, D. R. & Walker, C. S., 28 Nov 2017, In : British Journal of Pharmacology. In press, 41 p.

Research output: Contribution to journalArticle

Open Access
File
Calcitonin Receptors
Calcitonin Gene-Related Peptide
Calcitonin
Calcitonin Receptor-Like Protein
Calcitonin Gene-Related Peptide Receptors
2016

An allosteric role for receptor activity-modifying proteins in defining GPCR pharmacology

Gingell, J. J., Simms, J., Barwell, J., Poyner, D. R., Watkins, H. A., Pioszak, AA., Sexton, P. M. & Hay, D. L., 17 May 2016, In : Cell Discovery. 2, 16012.

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Proteins
Calcitonin Receptors
Pharmacology
G-Protein-Coupled Receptors
Islet Amyloid Polypeptide

GPCR-styrene maleic acid lipid particles (GPCR-SMALPs): their nature and potential

Wheatley, M., Charlton, J., Jamshad, M., Routledge, S. J., Bailey, S., La-Borde, P. J., Azam, M. T., Logan, R. T., Bill, R. M., Dafforn, T. R. & Poyner, D. R., 15 Apr 2016, In : Biochemical Society Transactions. 44, 2, p. 619-623 5 p.

Research output: Contribution to journalReview article

Open Access
File
Styrene
G-Protein-Coupled Receptors
Detergents
Lipids
Membranes

Receptor activity-modifying protein-directed G protein signaling specificity for the calcitonin gene-related peptide family of receptors

Weston, C., Winfield, I., Harris, M., Hodgson, R., Shah, A., Dowell, S. J., Mobarec, J. C., Woodlock, D. A., Reynolds, C. A., Poyner, D. R., Watkins, H. A. & Ladds, G., 14 Oct 2016, In : Journal of Biological Chemistry. 291, 42, p. 21925-21944 20 p.

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Proteins
Calcitonin Gene-Related Peptide Receptors
Calcitonin Gene-Related Peptide
GTP-Binding Proteins
Calcitonin Receptor-Like Protein

Receptor activity-modifying proteins; multifunctional G protein-coupled receptor accessory proteins

Hay, D. L., Walker, C. S., Gingell, J. J., Ladds, G., Reynolds, C. A. & Poyner, D. R., 15 Apr 2016, In : Biochemical Society Transactions. 44, 2, p. 568-573 6 p.

Research output: Contribution to journalReview article

Receptor Activity-Modifying Proteins
Accessories
G-Protein-Coupled Receptors
Calcitonin Receptor-Like Protein
Proteins

Receptor activity-modifying proteins 2 and 3 generate adrenomedullin receptor subtypes with distinct molecular properties

Watkins, H. A., Chakravarthy, M., Abhayawardana, R. S., Gingell, J. J., Garelja, M., Pardamwar, M., McElhinney, J. M. W. R., Lathbridge, A., Constantine, A., Harris, P. W. R., Yuen, T-Y., Brimble, M. A., Barwell, J., Poyner, D. R., Woolley, M. J., Conner, A. C., Pioszak, AA., Reynolds, C. A. & Hay, D. L., 27 May 2016, In : Journal of Biological Chemistry. 291, 22, p. 11657-11675 19 p.

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Protein 3
Receptor Activity-Modifying Protein 2
Calcitonin Receptor-Like Protein
Adrenomedullin Receptors
Adrenomedullin
2015

G-protein-coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent

Jamshad, M., Charlton, J., Lin, Y-P., Routledge, S. J., Bawa, Z., Knowles, T. J., Overduin, M., Dekker, N., Dafforn, T. R., Bill, R. M., Poyner, D. R. & Wheatley, M., 27 Feb 2015, In : Bioscience Reports. 35, 2, 10 p., e00188.

Research output: Contribution to journalArticle

Open Access
File
G-Protein-Coupled Receptors
Detergents
Purification
Lipids
Styrene

Modulation of glucagon receptor pharmacology by Receptor Activity-modifying Protein-2 (RAMP2)

Weston, C., Lu, J., Li, N., Barkan, K., Richards, G. O., Roberts, D. J., Skerry, T. M., Poyner, D., Pardamwar, M., Reynolds, C. A., Dowell, S. J., Willars, G. B. & Ladds, G., 18 Sep 2015, In : Journal of Biological Chemistry. 290, 38, p. 23009-23022 14 p.

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Protein 2
Glucagon Receptors
Modulation
Pharmacology
Receptor-Interacting Protein Serine-Threonine Kinases

Structural basis for receptor activity-modifying protein-dependent selective peptide recognition by a G protein-coupled receptor

Booe, JM., Walker, C. S., Barwell, J., Kuteyi, G., Simms, J., Jamaluddin, MA., Warner, ML., Bill, R. M., Harris, PW., Brimble, MA., Poyner, D. R., Hay, D. L. & Pioszak, AA., 18 Jun 2015, In : Molecular Cell. 58, 6, p. 1040-1052 13 p.

Research output: Contribution to journalArticle

Open Access
File
Receptor Activity-Modifying Proteins
Calcitonin Receptor-Like Protein
G-Protein-Coupled Receptors
Peptides
Adrenomedullin